ARG63263
anti-Npap60 / Nup50 antibody
anti-Npap60 / Nup50 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Npap60 / Nup50 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Npap60 / Nup50 |
| 抗原物种 | Human |
| 抗原 | C-ELHKILLEKKDA |
| 偶联标记 | Un-conjugated |
| 別名 | Nuclear pore complex protein Nup50; 50 kDa nucleoporin; NPAP60L; Nucleoporin Nup50; NPAP60; Nuclear pore-associated protein 60 kDa-like |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9UKX7 Human Nuclear pore complex protein Nup50 |
|---|---|
| 背景介绍 | The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 50 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG63263 anti-Npap60 / Nup50 antibody WB image
Western Blot: Jurkat lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63263 anti-Npap60 / Nup50 antibody at 0.5 µg/ml dilution.
-
ARG63263 anti-Npap60 / Nup50 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Uterus. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63263 anti-Npap60 / Nup50 antibody at 3.8 µg/ml dilution followed by AP-staining.
