ARG54245
anti-Nitrotyrosine antibody [EM-30]
anti-Nitrotyrosine antibody [EM-30] for IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and All
Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [EM-30] recognizes Nitrotyrosine |
|---|---|
| 反应物种 | All |
| 应用 | IHC-Fr, IHC-P, WB |
| 特异性 | The clone EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | EM-30 |
| 同位型 | IgG1 |
| 靶点名称 | Nitrotyrosine |
| 抗原 | NO2-Tyr-CH2-Thyroglobulin |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | WB: NO2-BSA and NO2-HSA IHC-P and IHC-Fr: Colon cancer epithelium, prostate hyperplasia |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage. |
|---|---|
| 研究领域 | Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
检测图片 (1) Click the Picture to Zoom In
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ARG54245 anti-Nitrotyrosine antibody [EM-30] IHC-Fr image
Immunohistochemistry: Human tumoral colon epithelium (frozen section) stained with antibody ARG54245 anti-Nitrotyrosine antibody [EM-30].
