ARG22250
anti-Neuroligin 1 antibody [S97A-31]
anti-Neuroligin 1 antibody [S97A-31] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [S97A-31] recognizes Neuroligin 1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | Detects ~120kDa. Does not cross-react with other Neuroligins. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | S97A-31 |
| 同位型 | IgG1 |
| 靶点名称 | Neuroligin 1 |
| 抗原物种 | Rat |
| 抗原 | Fusion protein around aa. 718-843 (cytoplasmic C-terminus) of Rat Neuroligin 1. Mouse: 99% identity (125/126 around aa. identical). Human: 99% identity (125/126 around aa. identical); >40% identity with Neuroligin-2 and -3. |
| 偶联标记 | Un-conjugated |
| 別名 | NL1; Neuroligin-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.1% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.1% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Nlgn1 |
| 全名 | neuroligin 1 |
| 背景介绍 | This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Required to maintain wakefulness quality and normal synchrony of cerebral cortex activity during wakefulness and sleep. [UniProt] |
| 细胞定位 | Cell Junction, Cell membrane, postsynaptic cell membrane, postsynaptic density, Synapse |
| 预测分子量 | 96 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG22250 anti-Neuroligin 1 antibody [S97A-31] ICC/IF image
Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22250 anti-Neuroligin 1 antibody [S97A-31] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ARG22250 anti-Neuroligin 1 antibody [S97A-31] (D) Composite.
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ARG22250 anti-Neuroligin 1 antibody [S97A-31] WB image
Western blot: 15 µg of Mouse Brain Membrane. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: ARG22250 anti-Neuroligin 1 antibody [S97A-31] at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT.
