ARG65051
anti-Neurogranin precursor antibody
anti-Neurogranin precursor antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Neuroscience antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Neurogranin precursor |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Cow, Dog |
| 应用 | IHC-P, WB |
| 特异性 | Reported variants represent identical protein: NP_006167.1, NP_001119653.1 |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Neurogranin precursor |
| 抗原物种 | Human |
| 抗原 | C-KIKSGERGRKG |
| 偶联标记 | Un-conjugated |
| 別名 | Neurogranin; 55-78; Ng; hng; RC3 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. [provided by RefSeq, Jul 2008] |
| 研究领域 | Neuroscience antibody |
| 预测分子量 | 8 kDa |
| 翻译后修饰 | Phosphorylated at Ser-36 by PHK and PKC, phosphorylation prevents interaction with Calmodulin and interrupts several learning- and memory-associated functions. |
检测图片 (4) Click the Picture to Zoom In
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ARG65051 anti-Neurogranin precursor antibody WB image
Western Blot: Mouse Brain lysate (35 µg protein in RIPA buffer) stained with ARG65051 anti-Neurogranin antibody at 0.1 µg/ml dilution.
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ARG65051 anti-Neurogranin precursor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65051 anti-Neurogranin precursor antibody at 5 µg/ml dilution followed by AP-staining.
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ARG65051 anti-Neurogranin precursor antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65051 anti-Neurogranin antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG65051 anti-Neurogranin precursor antibody WB image
Western blot: 35 µg of Human brain (frontal cortex) lysate (in RIPA buffer) stained with ARG65051 anti-Neurogranin precursor antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
