ARG56066
anti-Neurofilament NF-L antibody [NR-4]
anti-Neurofilament NF-L antibody [NR-4] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Rat
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody
概述
| 产品描述 | Mouse Monoclonal antibody [NR-4] recognizes Neurofilament NF-L |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | FACS, ICC/IF, IHC-P |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | NR-4 |
| 同位型 | IgG1 |
| 靶点名称 | Neurofilament NF-L |
| 抗原物种 | Pig |
| 抗原 | Crude neurofilament preparation from Pig spinal cord. |
| 偶联标记 | Un-conjugated |
| 別名 | Neurofilament triplet L protein; 68 kDa neurofilament protein; CMT1F; NF68; NFL; CMT2E; Neurofilament light polypeptide; NF-L; PPP1R110 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NEFL |
| 全名 | neurofilament, light polypeptide |
| 背景介绍 | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008] |
| 生物功能 | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. [UniProt] |
| 细胞定位 | Cytoplasmic |
| 产品亮点 | Related products: Neurofilament NF L antibodies; Neurofilament NF L Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Neuronal Development Marker |
| 研究领域 | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody |
| 预测分子量 | 62 kDa |
| 翻译后修饰 | O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1. |
检测图片 (2) Click the Picture to Zoom In
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ARG56066 anti-Neurofilament NF-L antibody [NR-4] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human cerebellum stained with ARG56066 anti-Neurofilament NF-L antibody [NR-4].
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ARG56066 anti-Neurofilament NF-L antibody [NR-4] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Rat cerebellum stained with ARG56066 anti-Neurofilament NF-L antibody [NR-4].
