ARG52349
anti-Neurofilament NF-L antibody
anti-Neurofilament NF-L antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody
概述
| 产品描述 | Chicken Polyclonal antibody recognizes Neurofilament NF-L |
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| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Chk |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Chicken |
| 克隆 | Polyclonal |
| 同位型 | IgY |
| 靶点名称 | Neurofilament NF-L |
| 抗原物种 | Bovine |
| 抗原 | Preparation of bovine spinal cord NF-L |
| 偶联标记 | Un-conjugated |
| 別名 | Neurofilament triplet L protein; 68 kDa neurofilament protein; CMT1F; NF68; NFL; CMT2E; Neurofilament light polypeptide; NF-L; PPP1R110 |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~ 68 kDa Neurofilament L protein in Western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 68 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Total IgY fraction |
| 缓冲液 | Total IgY fraction in PBS and 10 mM Sodium azide |
| 抗菌剂 | 10 mM Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NEFL |
| 全名 | neurofilament, light polypeptide |
| 背景介绍 | Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H . NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease . |
| 生物功能 | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. [UniProt] |
| 研究领域 | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody |
| 预测分子量 | 62 kDa |
| 翻译后修饰 | O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1. |
检测图片 (4) Click the Picture to Zoom In
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ARG52349 anti-Neurofilament NF-L antibody ICC/IF image
Immunofluorescence: Mixed cultured rat neurons and glia stained with ARG52349 anti-Neurofilament NF-L antibody (red) at 1:5000 dilution.
The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites, and perikarya.
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ARG52349 anti-Neurofilament NF-L antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat cerebellum tissue stained with ARG52349 anti-Neurofilament NF-L antibody (green) at 1:2000 dilution, and co-stained with ARG52283 anti-FOX3 / NeuN antibody [1B7] (red) at 1:5000 dilution. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.)
The NF-L antibody labels perikarya and processes of neuronal cells, particularly strongly the axons of basket cells, while the FOX3 / NeuN antibody stains the nuclei and proximal cytoplasm of neurons.
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ARG52349 anti-Neurofilament NF-L antibody WB image
Western blot: Rat brain, Rat spinal cord, Mouse brain, Mouse spinal cord and Cow spinal cord lysates stained with ARG52349 anti-Neurofilament NF-L antibody (green) at 1:20000 dilution.
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ARG52349 anti-Neurofilament NF-L antibody WB image
Western blot: Rat cortex lysate stained with ARG52349 anti-Neurofilament NF-L antibody.
