ARG10761
anti-Neurofilament NF-H antibody
anti-Neurofilament NF-H antibody for ICC/IF,IHC-Frozen sections,Western blot and Mouse,Rat
Neuroscience antibody; Signaling Transduction antibody; Neurofilament antibody; Intermediate Neurofilament antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Neurofilament NF-H |
|---|---|
| 反应物种 | Ms, Rat |
| 预测物种 | Hu, Bov, Hrs, Pig |
| 应用 | ICC/IF, IHC-Fr, WB |
| 特异性 | This antibody reacts very strongly with NF-H KSP phosphorylated repeats. Reactivity with non-phosphorylated KSP sequences is orders of magnitude weaker. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Neurofilament NF-H |
| 抗原物种 | Bovine |
| 抗原 | Native NF-H purified from Bovine spinal cord. |
| 偶联标记 | Un-conjugated |
| 別名 | Neurofilament heavy polypeptide; 200 kDa neurofilament protein; NF-H; Neurofilament triplet H protein; NFH |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Unpurified. |
| 缓冲液 | Serum. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NEFH |
| 全名 | neurofilament, heavy polypeptide |
| 背景介绍 | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008] |
| 生物功能 | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. [UniProt] |
| 研究领域 | Neuroscience antibody; Signaling Transduction antibody; Neurofilament antibody; Intermediate Neurofilament antibody |
| 预测分子量 | 112 kDa |
| 翻译后修饰 | There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. |
检测图片 (4) Click the Picture to Zoom In
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ARG10761 anti-Neurofilament NF-H antibody ICC/IF image
Immunocytochemistry: Rat Mixed neuron / glia cultures stained with ARG10761 anti-Neurofilament NF-H antibody (green) and co-stained with chicken antibody to neurofilament subunit NF-L (red). Axons contain phosphorylated NF-H and NF-L so appear yellow, while dendrites and perikarya only contain NF-L and so appear red. DNA is shown in blue.
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ARG10761 anti-Neurofilament NF-H antibody IHC-Fr image
Immunohistochemistry: Frozen section of Mouse hippocampus stained with ARG10761 anti-Neurofilament NF-H antibody (red) at 1:2000 dilution and costained with ARG10723 anti-Myelin Basic Protein antibody [7G7] (green) at 1:5000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.)
The NF-H antibody labels a network of axons of different neurons, while the Myelin Basic Protein antibody stains myelin sheath around these axons.
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ARG10761 anti-Neurofilament NF-H antibody WB image
Western blot: 20 µg of Rat brain lysate stained with ARG10761 anti-Neurofilament NF-H antibody at 1:25,000 dilution. A prominent band at 200 kDa corresponds to phosphorylated form of NF-H.
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ARG10761 anti-Neurofilament NF-H antibody WB image
Western blot: Rat brain, Rat spinal cord, Mouse brain and Mouse spinal cord lysates stained with ARG10761 anti-Neurofilament NF-H antibody (green) at 1:10000 dilution.
Strong band at about 220 kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected.
