ARG56065

anti-Neurofilament NF-H (Phosphorylated) antibody [NE14]

anti-Neurofilament NF-H (Phosphorylated) antibody [NE14] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Neuroscience antibody; Signaling Transduction antibody; Neurofilament antibody; Intermediate Neurofilament antibody

概述

产品描述 Mouse Monoclonal antibody [NE14] recognizes Neurofilament NF-H (Phosphorylated)
反应物种 Hu, Ms, Rat
应用 FACS, IHC-P, WB
宿主 Mouse
克隆 Monoclonal
克隆号 NE14
同位型 IgG1
靶点名称 Neurofilament NF-H
抗原物种 Pig
抗原 Crude neurofilament preparation from Pig spinal cord.
偶联标记 Un-conjugated
別名 Neurofilament heavy polypeptide; 200 kDa neurofilament protein; NF-H; Neurofilament triplet H protein; NFH

应用说明

应用建议
应用 推荐稀释比
FACS0.5 - 1 µg/10^6 cells
IHC-P2 - 5 µg/ml
WB1 - 2 µg/ml
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Human brain

属性

形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
抗菌剂 0.05% Sodium azide
稳定剂 0.1 mg/ml BSA
浓度 0.2 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 380684 Mouse NEFH

GeneID: 4744 Human NEFH

Swiss-port # P12036 Human Neurofilament heavy polypeptide

Swiss-port # P19246 Mouse Neurofilament heavy polypeptide

基因名称 NEFH
全名 neurofilament, heavy polypeptide
背景介绍 Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]
生物功能 Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. [UniProt]
细胞定位 Cytoplasmic
研究领域 Neuroscience antibody; Signaling Transduction antibody; Neurofilament antibody; Intermediate Neurofilament antibody
预测分子量 112 kDa
翻译后修饰 There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.

检测图片 (4) Click the Picture to Zoom In

  • ARG56065 anti-Neurofilament NF-H (Phosphorylated) antibody [NE14] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human cerebellum stained with ARG56065 anti-Neurofilament NF-H (Phosphorylated) antibody [NE14].

  • ARG56065 anti-Neurofilament NF-H (Phosphorylated) antibody [NE14] WB image

    Western blot: Human brain lysate stained with ARG56065 anti-Neurofilament NF-H (Phosphorylated) antibody [NE14].

  • ARG56065 anti-Neurofilament NF-H (Phosphorylated) antibody [NE14] FACS image

    Flow Cytometry: Permeabilized HEK293 cells stained with ARG56065 anti-Neurofilament NF-H (Phosphorylated) antibody [NE14] (blue); Isotype control (red).

  • ARG56065 anti-Neurofilament NF-H (Phosphorylated) antibody [NE14] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat cerebellum stained with ARG56065 anti-Neurofilament NF-H (Phosphorylated) antibody [NE14].