ARG52345
anti-Nestin antibody [4D11]
anti-Nestin antibody [4D11] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
参考文献3
概述
| 产品描述 | Mouse Monoclonal antibody [4D11] recognizes Nestin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Chk, Cow, Hrs, Pig |
| 应用 | ICC/IF, IHC-Fr, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4D11 |
| 同位型 | IgG1 |
| 靶点名称 | Nestin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around aa. 317-630 of Human Nestin. |
| 偶联标记 | Un-conjugated |
| 別名 | Nestin; Nbla00170 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | Specific for the ~220-240 kDa nestin doublet in Western blot in neonatal Rat brain homogenate. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Ascites fluid |
| 缓冲液 | Ascites fluid and 0.01 % Sodium azide |
| 抗菌剂 | 0.01 % Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NES |
| 全名 | nestin |
| 背景介绍 | Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells (Lendahl et al., 1990). Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embryo day 15 rat spinal cord (Hockfield et al., 1985)). One of these antibodies, called Rat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound. The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits. |
| 产品亮点 | Related Antibody Duos and Panels: ARG30006 NSC and Astrocyte Marker Antibody Duo (GFAP, Nestin) ARG30008 Neuron Development Marker Antibody Duo (Nestin, Tuj1) ARG30009 NSC and Neuron Marker Antibody Duo (Nestin, MAP2) ARG30146 Neural Stem / Progenitor Cell Marker Antibody Duo (Nestin, Vimentin) Related products: Nestin antibodies; Nestin Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: 14-3-3η as a promising target for the treatment of Major Depression Disorder Stem cell and the regenerative medicine: Ready for the patients Neuronal Development Marker Neuronal Development Marker Choose the Best ZIKA Virus Antibodies Fight microcephaly with arigo |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody |
| 预测分子量 | 177 kDa |
| 翻译后修饰 | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity). |
检测图片 (4) Click the Picture to Zoom In
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ARG52345 anti-Nestin antibody [4D11] ICC/IF image
Immunofluorescence: Cultured neonatal Rat neurons and glia showing nestin labeling in red (ARG52345 Nestin antibody [4D11]) and vimentin (ARG52468 anti-Vimentin antibody) in green.
Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
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ARG52345 anti-Nestin antibody [4D11] ICC/IF image
Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG52345 anti-Nestin antibody [4D11] (red) at 1:500 dilution and costained with ARG52328 anti-MAP2 antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining.
The Nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
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ARG52345 anti-Nestin antibody [4D11] WB image
Western blot: Rat brain (P18) homogenate stained with ARG52345 anti-Nestin antibody [4D11].
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ARG52345 anti-Nestin antibody [4D11] WB image
Western blot: Rat brain (embryonic, E18), C6 and SH-SY5Y cell lysates stained with ARG52345 anti-Nestin antibody [4D11] (green) at 1:500 dilution.
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