ARG43798
anti-Nephrin phospho (Tyr1217) antibody
anti-Nephrin phospho (Tyr1217) antibody for Western blot and Human
Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Nephrin |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Nephrin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to internal region of human nephrin. |
| 偶联标记 | Un-conjugated |
| 別名 | CNF; NPHN; Renal glomerulus-specific cell adhesion receptor; nephrin; Nephrin |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | A549 | ||||
| 实际分子量 | ~135 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NPHS1 |
| 全名 | nephrosis 1, congenital, Finnish type (nephrin) |
| 背景介绍 | This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009] |
| 生物功能 | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). [UniProt] |
| 细胞定位 | Membrane |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 135 kDa |
| 翻译后修饰 | Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. |
检测图片 (1) Click the Picture to Zoom In
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ARG43798 anti-Nephrin phospho (Tyr1217) antibody WB image
Western blot: A549 cell lysates stained with anti-Nephrin phospho (Tyr1217) antibody.
