ARG65357
anti-Neisseria meningitidis antigen Orf 1 / FrpD antibody
anti-Neisseria meningitidis antigen Orf 1 / FrpD antibody for Western blot and Bacteria
Microbiology and Infectious Disease antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Neisseria meningitidis antigen Orf 1 / FrpD |
|---|---|
| 反应物种 | Bacteria |
| 应用 | WB |
| 特异性 | The polyclonal antibody reacts with Orf1 (FrpD), a 28 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Neisseria meningitidis antigen Orf 1 / FrpD |
| 抗原 | Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | WB: Under reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from rabbit serum by protein-A afinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane. |
| 研究领域 | Microbiology and Infectious Disease antibody |
检测图片 (1) Click the Picture to Zoom In
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ARG65357 anti-Neisseria meningitidis antigen Orf 1 / FrpD antibody WB image
Western blot: Recombinant Orf1 proteins stained with ARG65357 anti-Neisseria meningitidis antigen Orf 1 / FrpD antibody.
Lane 1, 3, 5: cell lysate of Orf1-transfected E. coli; dilution (1) 0.5 µg/ml, (3) 0.2 µg/ml, (5) 0.1 µg/ml; Lane 2, 4, 6: cell lysates of non-transfected E. coli (negative controls).
