ARG44115

anti-NUP54 antibody

anti-NUP54 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal recognizes NUP54
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 NUP54
抗原物种 Human
抗原 Human NUP54 recombinant protein (Position: Q166-H493).
偶联标记 Un-conjugated
別名 NUP54; Nucleoporin 54; 54 KDa Nucleoporin; Nucleoporin 54kDa; Nucleoporin P54; Nucleoporin 54kD; DYT37

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.1-0.25 µg/ml
应用说明 The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂 0.05% Sodium azide
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 269113 Mouse NUP54

GeneID: 53371 Human NUP54

GeneID: 53372 Rat NUP54

基因名称 NUP54
全名 Nucleoporin 54
背景介绍 The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the phe-gly (FG) repeat-containing nucleoporin subset. Multiple alternatively spliced transcript variants have been found for this gene.
生物功能 Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane.
细胞定位 Membrane, Nuclear pore complex, Nucleus
预测分子量 55 kDa
翻译后修饰 Glycoprotein

检测图片 (6) Click the Picture to Zoom In

  • ARG44115 anti-NUP54 antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG44115 anti-NUP54 antibody at 5 μg/ml dilution.

  • ARG44115 anti-NUP54 antibody WB image

    Western blot: Hela, K562, HepG2 and A549 stained with ARG44115 anti-NUP54 antibody at 0.25 μg/ml dilution.

  • ARG44115 anti-NUP54 antibody FACS image

    Flow Cytometry: HeLa stained with ARG44115 anti-NUP54 antibody at 1 µg/10^6 cells dilution.

  • ARG44115 anti-NUP54 antibody IHC-P image

    Immunohistochemistry: Rat brain stained with ARG44115 anti-NUP54 antibody at 2 μg/ml dilution.

  • ARG44115 anti-NUP54 antibody WB image

    Western blot: RH35 stained with ARG44115 anti-NUP54 antibody at 0.25 μg/ml dilution.

  • ARG44115 anti-NUP54 antibody WB image

    Western blot: NIH/3T3 stained with ARG44115 anti-NUP54 antibody at 0.25 μg/ml dilution.