ARG40146

anti-NSFL1C antibody

anti-NSFL1C antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

概述

产品描述 Rabbit Polyclonal antibody recognizes NSFL1C
反应物种 Hu, Ms
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 NSFL1C
抗原物种 Human
抗原 KLH-conjugated synthetic peptide between aa. 214-248 of Human NSFL1C.
偶联标记 Un-conjugated
別名 p97 cofactor p47; dJ776F14.1; UBXD10; UBX domain-containing protein 2C; NSFL1 cofactor p47; UBX1; UBXN2C; P47

应用说明

应用建议
应用 推荐稀释比
IHC-P1:25
WB1:2000
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 293

属性

形式 Liquid
纯化 Purification with Protein A and immunogen peptide.
缓冲液 PBS and 0.09% (W/V) Sodium azide.
抗菌剂 0.09% (W/V) Sodium azide.
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 386649 Mouse NSFL1C

GeneID: 55968 Human NSFL1C

Swiss-port # Q9CZ44 Mouse NSFL1 cofactor p47

Swiss-port # Q9UNZ2 Human NSFL1 cofactor p47

基因名称 NSFL1C
全名 NSFL1 (p97) cofactor (p47)
背景介绍 N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011]
生物功能 Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro). [UniProt]
细胞定位 Nucleus. Golgi apparatus, Golgi stack. Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks. Localizes to centrosome during mitotic prophase and metaphase. [UniProt]
预测分子量 41 kDa
翻译后修饰 Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis (By similarity). [UniProt]

检测图片 (2) Click the Picture to Zoom In

  • ARG40146 anti-NSFL1C antibody IHC-P image

    Immunohistochemistry: Paraformaldehyde-fixed and paraffin-embedded Human testis was blocked with 3% BSA for 0.5 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG40146 anti-NSFL1C antibody at 1:25 for 1 hour at 37°C.

  • ARG40146 anti-NSFL1C antibody WB image

    Western blot: 20 µg of 293 cell lysate stained with ARG40146 anti-NSFL1C antibody at 1:2000 dilution.