ARG42028

anti-NSF antibody

anti-NSF antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes NSF
反应物种 Hu, Ms, Rat
应用 ICC/IF, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 NSF
抗原物种 Human
抗原 Recombinant fusion protein corresponding to aa. 1-250 of Human NSF (NP_006169.2).
偶联标记 Un-conjugated
別名 SKD2; N-ethylmaleimide-sensitive fusion protein; Vesicular-fusion protein NSF; Vesicle-fusing ATPase; NEM-sensitive fusion protein; EC 3.6.4.6

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:500 - 1:2000
IPAssay-dependent
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Rat brain
实际分子量 ~ 80 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 18195 Mouse NSF

GeneID: 4905 Human NSF

GeneID: 60355 Rat NSF

基因名称 NSF
全名 N-ethylmaleimide-sensitive factor
生物功能 Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity). [UniProt]
细胞定位 Cytoplasm. [UniProt]
预测分子量 83 kDa
翻译后修饰 Phosphorylation at Ser-569 interferes with homohexamerization. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG42028 anti-NSF antibody WB image

    Western blot: 25 µg of Rat brain lysate stained with ARG42028 anti-NSF antibody at 1:1000 dilution.