ARG66878
anti-NSE / Neuron Specific Enolase antibody [SQab22281]
anti-NSE / Neuron Specific Enolase antibody [SQab22281] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [SQab22281] recognizes NSE / Neuron Specific Enolase |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SQab22281 |
| 同位型 | IgG |
| 靶点名称 | NSE / Neuron Specific Enolase |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within Human NSE aa 416-433. |
| 偶联标记 | Un-conjugated |
| 別名 | Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC 4.2.1.11 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | 47 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ENO2 |
| 全名 | enolase 2 (gamma, neuronal) |
| 背景介绍 | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008] |
| 生物功能 | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt] |
| 产品亮点 | Related products: NSE antibodies; Anti-Mouse IgG secondary antibodies; Related news: Neuronal Development Marker |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 47 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG66878 anti-NSE antibody [SQab22281] IHC-P image
Immunohistochemistry: Paraffin-embedded Neuroblastoma stained with ARG66878 anti-NSE antibody [SQab22281] at 1:200.
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ARG66878 anti-NSE / Neuron Specific Enolase antibody [SQab22281] WB image (Customer review)
Western blot: HepG2 stained with ARG66878 anti-NSE / Neuron Specific Enolase antibody [SQab22281] at 1:500 dilution.
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ARG66878 anti-NSE / Neuron Specific Enolase antibody [SQab22281] WB image (Customer review)
Western blot: Mouse brain stained with ARG66878 anti-NSE / Neuron Specific Enolase antibody [SQab22281] at 1:500 dilution.
客户反馈
Excellent
anti-NSE / Neuron Specific Enolase antibody [SQab22281]
Application：WB
Sample：Mouse Brain
Sample Loading Amount：10 μL
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
anti-NSE / Neuron Specific Enolase antibody [SQab22281]
Application：WB
Sample：HepG2
Sample Loading Amount：10 μL
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
