ARG43621
anti-NSE / Neuron Specific Enolase antibody
anti-NSE / Neuron Specific Enolase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NSE / Neuron Specific Enolase |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NSE / Neuron Specific Enolase |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within a.a. 50-150 of Human NSE / Neuron Specific Enolase. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Gamma-enolase |
| 別名 | Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC 4.2.1.11 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 48 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ENO2 |
| 全名 | enolase 2 (gamma, neuronal) |
| 背景介绍 | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008] |
| 生物功能 | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. [UniProt] |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | Acetylation; Hydroxylation; Isopeptide bond; Phosphoprotein; Ubl conjugation |
检测图片 (7) Click the Picture to Zoom In
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ARG43621 anti-NSE / Neuron Specific Enolase antibody WB image
Western blot: A431 and HepG2 stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 0.5 μg/mL dilution.
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ARG43621 anti-NSE / Neuron Specific Enolase antibody IHC-P image
Immunohistochemistry: Human pancreatic cancer stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 1 μg/ml dilution.
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ARG43621 anti-NSE / Neuron Specific Enolase antibody ICC/IF image
Immunofluorescence: A431 stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 5 μg/ml dilution.
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ARG43621 anti-NSE / Neuron Specific Enolase antibody FACS image
Flow Cytometry: A431 stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 1 μg / 10^6 cells dilution.
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ARG43621 anti-NSE / Neuron Specific Enolase antibody WB image
Western blot: Rat brain and Mouse brain stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 0.5 μg/mL dilution.
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ARG43621 anti-NSE / Neuron Specific Enolase antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 1 μg/ml dilution.
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ARG43621 anti-NSE / Neuron Specific Enolase antibody IHC-P image
Immunohistochemistry: Mouse brain stained with ARG43621 anti-NSE / Neuron Specific Enolase antibody at 1 μg/ml dilution.
