ARG57125
anti-NSE / Neuron Specific Enolase antibody [17D10]
anti-NSE / Neuron Specific Enolase antibody [17D10] for ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [17D10] recognizes NSE / Neuron Specific Enolase |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 17D10 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | NSE / Neuron Specific Enolase |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-434 of Human NSE |
| 偶联标记 | Un-conjugated |
| 別名 | Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC 4.2.1.11 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ENO2 |
| 全名 | enolase 2 (gamma, neuronal) |
| 背景介绍 | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008] |
| 生物功能 | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt] |
| 预测分子量 | 47 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG57125 anti-NSE / Neuron Specific Enolase antibody [17D10] ICC/IF image
Immunofluorescence: U87MG cells line stained with ARG57125 anti-NSE / Neuron Specific Enolase antibody [17D10] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57125 anti-NSE / Neuron Specific Enolase antibody [17D10] WB image
Western blot: 40 µg of Mouse brain lysate stained with ARG57125 anti-NSE / Neuron Specific Enolase antibody [17D10] at 1) 1:500, 2) 1:1000, and 3) 1:5000.
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ARG57125 anti-NSE / Neuron Specific Enolase antibody [17D10] WB image
Western blot: 5 µg of 1) 293T, and 2) NSE transfected 293T cell lysate stained with ARG57125 anti-NSE / Neuron Specific Enolase antibody [17D10] at 1:1000.
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ARG57125 anti-NSE / Neuron Specific Enolase antibody [17D10] WB image
Western blot: 20 µg of 1) U87 MG, and 2) Jurkat cell lysates stained with ARG57125 anti-NSE / Neuron Specific Enolase antibody [17D10] at 1:1000.
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ARG57125 anti-NSE / Neuron Specific Enolase antibody [17D10] WB image
Western blot: 50 ng of 1) ENO1, 2) ENO2, and 3) ENO3 recombinant proteins stained with ARG57125 anti-NSE / Neuron Specific Enolase antibody [17D10] at 1:1000.
