ARG64848
anti-NR1I2 / PXR antibody
anti-NR1I2 / PXR antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes NR1I2 / PXR |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognize all reported isoforms (NP_071285.1; NP_148934.1; NP_003880.3). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NR1I2 / PXR |
| 抗原物种 | Human |
| 抗原 | EQFAITLKSYIECNR |
| 偶联标记 | Un-conjugated |
| 別名 | PAR; ONR1; PARq; Steroid and xenobiotic receptor; Nuclear receptor subfamily 1 group I member 2; PXR; BXR; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; SXR; PAR2; PAR1; PRR; SAR; Pregnane X receptor |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O75469 Human Nuclear receptor subfamily 1 group I member 2 |
|---|---|
| 背景介绍 | This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized. [provided by RefSeq, Jul 2008] |
| 研究领域 | Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 50 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG64848 anti-NR1I2 / PXR antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64848 anti-NR1I2 / PXR antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64848 anti-NR1I2 / PXR antibody WB image
Western blot: 35 µg of HepG2 lysate (in RIPA buffer) stained with ARG64848 anti-NR1I2 / PXR antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
