ARG63739
anti-NPM1 / Nucleophosmin antibody
anti-NPM1 / Nucleophosmin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Nucleolar Marker antibody; GC Marker antibody; Granular Component Marker antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes NPM1 / Nucleophosmin |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Dog |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | This antibody is expected to recognise both report isoforms (NP_002511.1; NP_954654.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NPM1 / Nucleophosmin |
| 抗原物种 | Human |
| 抗原 | C-QEAIQDLWQWRKSL |
| 偶联标记 | Un-conjugated |
| 別名 | NPM; Nucleolar protein NO38; B23; Nucleophosmin; Numatrin; Nucleolar phosphoprotein B23 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009] |
| 研究领域 | Nucleolar Marker antibody; GC Marker antibody; Granular Component Marker antibody |
| 预测分子量 | 33 kDa |
| 翻译后修饰 | Acetylated at C-terminal lysine residues, thereby increasing affinity to histones. ADP-ribosylated. Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels. Sumoylated by ARF. May be ubiquitinated. Ubiquitination leads to proteasomal degradation. |
检测图片 (4) Click the Picture to Zoom In
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ARG63739 anti-NPM1 / Nucleophosmin antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63739 anti-NPM1 / Nucleophosmin antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63739 anti-NPM1 / Nucleophosmin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63739 anti-NPM1 / Nucleophosmin antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63739 anti-NPM1 / Nucleophosmin antibody WB image
Western blot: 35 µg of NIH/3T3 (A), Daudi (B), Jurkat (C) and K562 (D) cell lysates (in RIPA buffer) stained with ARG63739 anti-NPM1 / Nucleophosmin antibody at 1 µg/ml (A), 0.1 µg/ml (B, C) and 0.01 µg/ml (D) dilutions and incubated at RT for 1 hour.
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ARG63739 anti-NPM1 / Nucleophosmin antibody WB image
Western blot: 35 µg of Mouse spleen lysate (in RIPA buffer) stained with ARG63739 anti-NPM1 / Nucleophosmin antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
