ARG64477

anti-NOVA1 antibody

anti-NOVA1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Neuroscience antibody

概述

产品描述 Goat Polyclonal antibody recognizes NOVA1
反应物种 Hu
应用 IHC-P, WB
特异性 This antibody is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1)
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 NOVA1
抗原物种 Human
抗原 C-REMPQNVAKTEPVS
偶联标记 Un-conjugated
別名 Paraneoplastic Ri antigen; Neuro-oncological ventral antigen 1; Nova-1; Onconeural ventral antigen 1; RNA-binding protein Nova-1; Ventral neuron-specific protein 1

应用说明

应用建议
应用 推荐稀释比
IHC-P5 µg/ml
WB0.01 - 0.03 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 4857 Human NOVA1

Swiss-port # P51513 Human RNA-binding protein Nova-1

背景介绍 This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
研究领域 Neuroscience antibody
预测分子量 52 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG64477 anti-NOVA1 antibody WB image

    Western Blot: Human Breast Cancer lysate (35 µg protein in RIPA buffer) stained with ARG64477 anti-NOVA1 antibody at 0.01 µg/ml dilution.

  • ARG64477 anti-NOVA1 antibody WB image

    Western Blot: 1). Mock transfection; 2) NOVA1 (RC210407) expressing plasmid transfected HEK293 cell lysate standed with ARG64477 anti-NOVA1 antibody

  • ARG64477 anti-NOVA1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64477 anti-NOVA1 antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG64477 anti-NOVA1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64477 anti-NOVA1 antibody at 5 µg/ml dilution followed by AP-staining.