ARG63870

anti-NOTCH3 antibody

Datasheet

订购信息

包装

价格 (USD)

100 μg

529

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

产品说明书

概述

产品描述	Goat Polyclonal antibody recognizes NOTCH3
反应物种	Hu
预测物种	Ms, Rat
应用	FACS, IHC-P
宿主	Goat
克隆	Polyclonal
同位型	IgG
靶点名称	NOTCH3
抗原物种	Human
抗原	C-QLGPQPEVTPKRQ
偶联标记	Un-conjugated
別名	LMNS; CADASIL; CASIL; Neurogenic locus notch homolog protein 3; IMF2; Notch 3

应用说明

应用建议

应用	推荐稀释比
FACS	10 µg/ml
IHC-P	4 - 6 µg/ml

应用说明

IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from goat serum by antigen affinity chromatography.
缓冲液	Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂	0.02% Sodium azide
稳定剂	0.5% BSA
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 4854 Human NOTCH3 Swiss-port # Q9UM47 Human Neurogenic locus notch homolog protein 3
背景介绍	This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008]
研究领域	Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody; Signaling Transduction antibody
预测分子量	244 kDa
翻译后修饰	Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). Phosphorylated. Hydroxylated by HIF1AN.

检测图片 (3) Click the Picture to Zoom In

ARG63870 anti-Notch 3 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63870 anti-Notch 3 antibody at 5 µg/ml dilution followed by AP-staining.
ARG63870 anti-Notch 3 antibody IHC-P image

Immunohistochemistry: Paraffin embedded Human Hippocampus. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63870 anti-Notch 3 antibody at 4 µg/ml dilution followed by HRP-staining.
ARG63870 anti-Notch 3 antibody FACS image

Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63870 anti-Notch 3 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).