ARG45460

anti-NOP58 antibody

anti-NOP58 antibody for Flow cytometry,ICC/IF,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes NOP58
反应物种 Hu
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 NOP58
抗原物种 Human
抗原 Recombinant protein containing to human NOP58.
偶联标记 Un-conjugated
別名 NOP58; NOP58 Ribonucleoprotein; NOP5; HSPC120; Nucleolar Protein 58; Nucleolar Protein 5; NOP58 Ribonucleoprotein Homolog (Yeast); NOP58 Ribonucleoprotein Homolog; Nucleolar Protein NOP5/NOP58; NOP5/NOP58; NOL5

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 70 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 51602 Human NOP58

Swiss-port # Q9Y2X3 Human Nucleolar protein 58

基因名称 NOP58
全名 NOP58 Ribonucleoprotein
背景介绍 The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs. [provided by RefSeq, Nov 2015]
生物功能 Required for 60S ribosomal subunit biogenesis. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles. Required for the biogenesis of box C/D snoRNAs such as U3, U8 and U14 snoRNAs. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. [UniProt]
细胞定位 Nucleus. [UniProt]
预测分子量 60 kDa
翻译后修饰 Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG45460 anti-NOP58 antibody ICC/IF image

    Immunofluorescence: CACO-2 stained with ARG45460 anti-NOP58 antibody at 5 μg/ml dilution.

  • ARG45460 anti-NOP58 antibody WB image

    Western blot: Hela stained with ARG45460 anti-NOP58 antibody at 0.5 μg/ml dilution.

  • ARG45460 anti-NOP58 antibody FACS image

    Flow Cytometry: U251 stained with ARG45460 anti-NOP58 antibody at 1 µg/10^6 cells dilution.