ARG63972
anti-NONO / p54nrb antibody
anti-NONO / p54nrb antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes NONO / p54nrb |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Dog, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NONO / p54nrb |
| 抗原物种 | Human |
| 抗原 | C-NRAAPGAEFAPNK |
| 偶联标记 | Un-conjugated |
| 別名 | Non-POU domain-containing octamer-binding protein; p54nrb; NonO protein; DNA-binding p52/p100 complex, 52 kDa subunit; P54NRB; NRB54; p54; NMT55; 55 kDa nuclear protein; 54 kDa nuclear RNA- and DNA-binding protein; P54; PPP1R114; nrb |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q15233 Human Non-POU domain-containing octamer-binding protein Swiss-port # Q99K48 Mouse Non-POU domain-containing octamer-binding protein |
|---|---|
| 背景介绍 | This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | The N-terminus is blocked. |
检测图片 (4) Click the Picture to Zoom In
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ARG63972 anti-NONO / p54nrb antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen (Steamed antigen retrieval with citrate buffer pH 6.0) stained with ARG63972 anti-NONO / p54nrb antibody at 3.8 µg/ml dilution, followed by AP-staining.
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ARG63972 anti-NONO / p54nrb antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63972 anti-NONO / p54nrb antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63972 anti-NONO / p54nrb antibody WB image
Western blot: 35 µg of Mouse brain lysate stained with ARG63972 anti-NONO / p54nrb antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG63972 anti-NONO / p54nrb antibody WB image
Western blot: 1) Mock transfection and 2) Human NONO expressing plasmid transfected HEK293 cell lysate stained with ARG63972 anti-NONO / p54nrb antibody.
