ARG40508
anti-NONO / p54nrb antibody
anti-NONO / p54nrb antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NONO / p54nrb |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Bov |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NONO / p54nrb |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 1-35 of Human NONO / p54nrb. (MQSNKTFNLEKQNHTPRKHHQHHHQQQHHQQQQQQ) |
| 偶联标记 | Un-conjugated |
| 別名 | Non-POU domain-containing octamer-binding protein; p54nrb; NonO protein; DNA-binding p52/p100 complex, 52 kDa subunit; P54NRB; NRB54; p54; NMT55; 55 kDa nuclear protein; 54 kDa nuclear RNA- and DNA-binding protein; P54; PPP1R114; nrb |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NONO |
| 全名 | non-POU domain containing, octamer-binding |
| 背景介绍 | This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009] |
| 生物功能 | DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. [UniProt] |
| 细胞定位 | Nucleus. Nucleus, nucleolus. Nucleus speckle. Note=Detected in punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. [UniProt] |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | The N-terminus is blocked. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG40508 anti-NONO / p54nrb antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain stained with ARG40508 anti-NONO / p54nrb antibody at 1 µg/ml dilution.
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ARG40508 anti-NONO / p54nrb antibody WB image
Western blot: Rat brain, Human placenta and PANC whole cell lysates stained with ARG40508 anti-NONO / p54nrb antibody at 0.5 µg/ml dilution.
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ARG40508 anti-NONO / p54nrb antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine stained with ARG40508 anti-NONO / p54nrb antibody at 1 µg/ml dilution.
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ARG40508 anti-NONO / p54nrb antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue stained with ARG40508 anti-NONO / p54nrb antibody at 1 µg/ml dilution.
