ARG45443
anti-NOLA1 antibody
anti-NOLA1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NOLA1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NOLA1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human NOLA1. |
| 偶联标记 | Un-conjugated |
| 別名 | GAR1; GAR1 Ribonucleoprotein; NOLA1; Nucleolar Protein Family A, Member 1 (H/ACA Small Nucleolar RNPs); H/ACA Ribonucleoprotein Complex Subunit 1; Nucleolar Protein Family A Member 1; SnoRNP Protein GAR1; GAR1 Ribonucleoprotein Homolog (Yeast); GAR1 Homolog, Ribonucleoprotein; GAR1 Ribonucleoprotein Homolog |
应用说明
| 应用建议 |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 25 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NY12 Human H/ACA ribonucleoprotein complex subunit 1 |
|---|---|
| 基因名称 | GAR1 |
| 全名 | GAR1 Ribonucleoprotein |
| 背景介绍 | This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 22 kDa |
| 翻译后修饰 | Isopeptide bond; Ubl conjugation. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
-
ARG45443 anti-NOLA1 antibody IHC-P image
Immunohistochemistry: Human gastric carcinoma stained with ARG45443 anti-NOLA1 antibody at 2 μg/ml dilution.
-
ARG45443 anti-NOLA1 antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45443 anti-NOLA1 antibody at 5 μg/ml dilution.
-
ARG45443 anti-NOLA1 antibody WB image
Western blot: A375 and K562 stained with ARG45443 anti-NOLA1 antibody at 0.5 μg/ml dilution.
-
ARG45443 anti-NOLA1 antibody FACS image
Flow Cytometry: K562 stained with ARG45443 anti-NOLA1 antibody at 1 µg/10^6 cells dilution.
