ARG52369
anti-NMDAR2B phospho (Tyr1472) antibody
anti-NMDAR2B phospho (Tyr1472) antibody for Western blot and Rat
Neuroscience antibody; Postsynaptic Receptor antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NMDAR2B phospho (Tyr1472) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Bov, Chk, Dog, NHuPrm, Zfsh |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NMDAR2B |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1472 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | MRD6; EIEE27; NR2B; hNR3; GluN2B; NR3; N-methyl D-aspartate receptor subtype 2B; Glutamate receptor ionotropic, NMDA 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl-D-aspartate receptor subunit 3; NMDAR2B |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~180k NMDAR NR2B-subunit protein phosphorylated at Tyr1472 in Western blots. The antibody also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either λ-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q00960 Rat Glutamate receptor ionotropic, NMDA 2B |
|---|---|
| 基因名称 | GRIN2B |
| 全名 | glutamate receptor, ionotropic, N-methyl D-aspartate 2B |
| 背景介绍 | The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity (Nakazawa et al., 2001; Roche et al., 2001). |
| 研究领域 | Neuroscience antibody; Postsynaptic Receptor antibody |
| 预测分子量 | 166 kDa |
| 翻译后修饰 | Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity. |
检测图片 (2) Click the Picture to Zoom In
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ARG52369 anti-NMDAR2B phospho (Tyr1472) antibody WB image
Western blot: Rat hippocampal lysate stained with ARG52369 anti-NMDAR2B phospho (Tyr1472) antibody showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472 (Control). Immunolabeling is blocked by treatment of the lysate with lambda phosphatase.
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ARG52369 anti-NMDAR2B phospho (Tyr1472) antibody WB image
Western blot: Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472 (Control) stained with ARG52369 anti-NMDAR2B phospho (Tyr1472) antibody.
