ARG22230
anti-NMDAR2B antibody [S59-36]
anti-NMDAR2B antibody [S59-36] for ICC/IF,Immunohistochemistry,IHC-Whole mount,Immunoprecipitation,Western blot and Human,Mouse,Rat,D. melanogaster
Neuroscience antibody; Postsynaptic Receptor antibody
参考文献1
概述
| 产品描述 | Mouse Monoclonal antibody [S59-36] recognizes NMDAR2B |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dm |
| 应用 | ICC/IF, IHC, IHC-Wmt, IP, WB |
| 特异性 | Detects ~166kDa. No cross-reactivity against NR2A. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | S59-36 |
| 同位型 | IgG2b |
| 靶点名称 | NMDAR2B |
| 抗原物种 | Rat |
| 抗原 | Fusion protein around aa. 20-271 (extracellular N-terminus) of Rat NMDAR2B |
| 偶联标记 | Un-conjugated |
| 別名 | MRD6; EIEE27; NR2B; hNR3; GluN2B; NR3; N-methyl D-aspartate receptor subtype 2B; Glutamate receptor ionotropic, NMDA 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl-D-aspartate receptor subunit 3; NMDAR2B |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Grin2b |
| 全名 | glutamate receptor, ionotropic, N-methyl D-aspartate 2B |
| 背景介绍 | N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008] |
| 生物功能 | NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity). [UniProt] |
| 细胞定位 | Cell Junction, Cell membrane, postsynaptic cell membrane, Synapse |
| 产品亮点 | Related products: anti-NMDAR2B antibody [S59-36] |
| 研究领域 | Neuroscience antibody; Postsynaptic Receptor antibody |
| 预测分子量 | 166 kDa |
| 翻译后修饰 | Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity. |
检测图片 (2) Click the Picture to Zoom In
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ARG22230 anti-NMDAR2B antibody [S59-36] WB image
Western blot: Rat brain membrane lysate stained with ARG22230 anti-NMDAR2B antibody [S59-36] at 1:1000 dilution.
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ARG22230 anti-NMDAR2B antibody [S59-36] WB image
Western blot: 20 µl of NR2B HEK-T lysates stained with ARG22230 anti-NMDAR2B antibody [S59-36] at 1:250 dilution (TBS for 60 min at RT). Block: 5% milk + TBST 1 hr at RT.
文献引用
Myc supercompetitor cells exploit the NMDA receptor to subdue their wild-type neighbours via cell competition.
IHC-Wmt / Drosophila
