ARG52363
anti-NMDAR2B antibody
anti-NMDAR2B antibody for Western blot and Mouse,Rat
Neuroscience antibody; Postsynaptic Receptor antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NMDAR2B |
|---|---|
| 反应物种 | Ms, Rat |
| 预测物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NMDAR2B |
| 抗原物种 | Rat |
| 抗原 | Fusion protein from the C-terminal region of the Rat NR2B subunit |
| 偶联标记 | Un-conjugated |
| 別名 | MRD6; Glutamate receptor ionotropic, NMDA 2B; hNR3; NR2B; EIEE27; GluN2B; N-methyl D-aspartate receptor subtype 2B; NR3; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl-D-aspartate receptor subunit 3; NMDAR2B |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~180k NR2B subunit of the NMDA receptor. Recognizes human, mouse and Rat forms of the NR2B subunits of NMDAR. Immunolabeling is blocked by preadsorption of antibody with the fusion protein used to geneRate the antibody. No reactivity towards the NR2A and NR2C subunits. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% Glycerol |
| 稳定剂 | 100 µg BSA per ml, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q00960 Rat Glutamate receptor ionotropic, NMDA 2B Swiss-port # Q01097 Mouse Glutamate receptor ionotropic, NMDA 2B |
|---|---|
| 基因名称 | GRIN2B |
| 全名 | glutamate receptor, ionotropic, N-methyl D-aspartate 2B |
| 背景介绍 | The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned and it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). |
| 产品亮点 | Related Antibody Duos and Panels: ARG30131 Postsynaptic Receptor Antibody Panel (NMDAR2A, NMDAR2B, GluR1) Related products: NMDAR2B antibodies; NMDAR2B Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Neuroscience antibody; Postsynaptic Receptor antibody |
| 预测分子量 | 166 kDa |
| 翻译后修饰 | Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity. |
检测图片 (1) Click the Picture to Zoom In
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ARG52363 anti-NMDAR2B antibody WB image
Western blot: 10 µg of Rat hippocampal (Hipp) lysate stained with ARG52363 anti-NMDAR2B antibody showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.
