ARG52356
anti-NMDAR1 N1 antibody
anti-NMDAR1 N1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Neuroscience antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NMDAR1 N1 |
|---|---|
| 反应物种 | Ms, Rat |
| 预测物种 | Hu, Chk, Dog, NHuPrm, Xenopus laevis |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NMDAR1 N1 |
| 抗原物种 | Rat |
| 抗原 | Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, N1 splice variant insert conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | NMDA1; GluN1; MRD8; NMD-R1; Glutamate receptor ionotropic, NMDA 1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NR1; NMDAR1 |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~120k NR1 subunit, N1 splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Powder |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | Lyophilized |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P35438 Mouse Glutamate receptor ionotropic, NMDA 1 Swiss-port # P35439 Rat Glutamate receptor ionotropic, NMDA 1 |
|---|---|
| 基因名称 | GRIN1 |
| 全名 | glutamate receptor, ionotropic, N-methyl D-aspartate 1 |
| 背景介绍 | The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory and neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR. |
| 研究领域 | Neuroscience antibody |
| 预测分子量 | 105 kDa |
| 翻译后修饰 | NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. |
检测图片 (1) Click the Picture to Zoom In
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ARG52356 anti-NMDAR1 N1 antibody WB image
Western blot: 10 μg of HEK 293 cells expressing:
Lane 1 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor containing the N1 splice variant insert.
Lane 2 - NR1 subunit containing only the C2 Insert;
Lane 3 - NR1 subunit containing the C1 and C2' Insert;
Lane 4 - without NR1 expression (Mock).
stained with ARG52356 anti-NMDAR1 N1 antibody.
