ARG55670
anti-NM23A antibody [1172CT2.4.1.1]
anti-NM23A antibody [1172CT2.4.1.1] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
概述
| 产品描述 | Mouse Monoclonal antibody recognizes NM23A |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1172CT2.4.1.1 |
| 同位型 | IgG2a |
| 靶点名称 | NM23A |
| 抗原物种 | Human |
| 抗原 | Purified His-tagged Human NM23A |
| 偶联标记 | Un-conjugated |
| 別名 | NDP kinase A; NDPK-A; NM23; Nucleoside diphosphate kinase A; Granzyme A-activated DNase; NDKA; NBS; NM23-H1; NB; NDPKA; NDK A; Tumor metastatic process-associated protein; EC 2.7.4.6; GAAD; AWD; Metastasis inhibition factor nm23 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Jurkat |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NME1 |
| 全名 | NME/NM23 nucleoside diphosphate kinase 1 |
| 背景介绍 | This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008] |
| 生物功能 | Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA |
| 预测分子量 | 17 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG55670 anti-NM23A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue stained with ARG55670 anti-NM23A antibody at 1:25 dilution.
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ARG55670 anti-NM23A antibody WB image
Western blot: 20 µg of Jurkat cell lysate stained with ARG55670 anti-NM23A antibody at 1:1000 dilution.
