概述

产品描述	Mouse Monoclonal antibody recognizes NLK
反应物种	Hu
应用	WB
宿主	Mouse
克隆	Monoclonal
克隆号	1146CT24.2.1
同位型	IgG2a
靶点名称	NLK
抗原物种	Human
抗原	Purified His-tagged NLK protein
偶联标记	Un-conjugated
別名	Protein LAK1; EC 2.7.11.24; Nemo-like kinase; Serine/threonine-protein kinase NLK

应用说明

应用建议	应用 推荐稀释比 WB 1:1000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	HeLa

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS and 0.09% (W/V) Sodium azide
抗菌剂	0.09% (W/V) Sodium azide
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 51701 Human NLK Swiss-port # Q9UBE8 Human Serine/threonine-protein kinase NLK
基因名称	NLK
全名	nemo-like kinase
生物功能	Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. [UniProt]
细胞定位	Nucleus. Cytoplasm. Note=Predominantly nuclear. A smaller fraction is cytoplasmic (By similarity).
预测分子量	58 kDa
翻译后修饰	Phosphorylated on Thr-298. Intermolecular autophosphorylation on Thr-298 activates the enzyme.

检测图片 (1) Click the Picture to Zoom In