ARG54322
anti-NIK antibody
anti-NIK antibody for ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NIK |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NIK |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa 931-947 of human NIK (accession no. Q99558). |
| 偶联标记 | Un-conjugated |
| 別名 | NIK; HSNIK; FTDCR1B; HS; EC 2.7.11.25; HsNIK; Serine/threonine-protein kinase NIK; NF-kappa-beta-inducing kinase; Mitogen-activated protein kinase kinase kinase 14 |
应用说明
| 应用建议 |
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| 应用说明 | Immunoblotting: 2 - 4 ug/ml. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | NIK-transfected 293 cells. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunoaffinity chroma-tography |
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q99558 Human Mitogen-activated protein kinase kinase kinase 14 |
|---|---|
| 基因名称 | MAP3K14 |
| 全名 | mitogen-activated protein kinase kinase kinase 14 |
| 背景介绍 | Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, LPS, and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNFα, and CD95 has been identified and designated NIK (for NF-κB inducing kinase). NIK is an activator of IκB kinase alpha and beta (IKKα and IKKβ). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the expression of a variety of gene products in response to pro-inflammatory cytokines and bacterial products. |
| 生物功能 | Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody |
| 预测分子量 | 104 kDa |
| 翻译后修饰 | Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization. Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. |
检测图片 (1) Click the Picture to Zoom In
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ARG54322 anti-NIK antibody ICC/IF image
Immunofluorescence: Hek293 cells stained with ARG54322 anti-NIK antibody at 20 μg/mL.
