ARG44061

anti-NHP2 antibody

anti-NHP2 antibody for Flow cytometry,ICC/IF,Western blot and Human

概述

产品描述 Rabbit Polyclonal recognizes NHP2
反应物种 Hu
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 NHP2
抗原物种 Human
抗原 Human NHP2 recombinant protein (Position: E21-Q136).
偶联标记 Un-conjugated
別名 NHP2P; H/ACA ribonucleoprotein complex subunit 2; Nucleolar protein family A member 2; NOLA2; DKCB2; snoRNP protein NHP2

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/1x10^6 cells
ICC/IF5 µg/ml
WB0.25 - 0.5 µg/ml
应用说明 The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂 0.05% Sodium azide
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 55651 Human NHP2

Swiss-port # Q9NX24 Human H/ACA ribonucleoprotein complex subunit 2

基因名称 NHP2
全名 NHP2 ribonucleoprotein
背景介绍 This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nhp2p. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]
生物功能 Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. [UniProt]
细胞定位 Nucleus, nucleolus. Nucleus, Cajal body. Note=Also localized to Cajal bodies (coiled bodies). [UniProt]
预测分子量 17 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG44061 anti-NHP2 antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG44061 anti-NHP2 antibody at 5 μg/mL dilution.

  • ARG44061 anti-NHP2 antibody WB image

    Western blot: SH-SY5Y, Caco-2 and Hela stained with ARG44061 anti-NHP2 antibody at 0.5 μg/mL dilution.

  • ARG44061 anti-NHP2 antibody FACS image

    Flow Cytometry: SH-SY5Y stained with ARG44061 anti-NHP2 antibody at 1 µg/1x10^6 cells dilution.