ARG66554

anti-NFYB antibody

anti-NFYB antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes NFYB
反应物种 Hu
应用 IHC-P
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 NFYB
抗原物种 Human
抗原 KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of Human NFYB.
偶联标记 Un-conjugated
別名 NF-YB; HAP3; Nuclear transcription factor Y subunit B; CBF-A; CAAT box DNA-binding protein subunit B; CBF-B; Nuclear transcription factor Y subunit beta

应用说明

应用建议
应用 推荐稀释比
IHC-P1:100 - 1:200
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 0.2% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.2% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 4801 Human NFYB

Swiss-port # P25208 Human Nuclear transcription factor Y subunit beta

基因名称 NFYB
全名 nuclear transcription factor Y, beta
背景介绍 The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]
生物功能 Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. [UniProt]
细胞定位 Nucleus. [UniProt]
预测分子量 23 kDa
翻译后修饰 Monoubiquitination at Lys-140 plays an important role in transcriptional activation by allowing the deposition of histone H3 methylations as well as histone H2B monoubiquitination at 'Lys-121'. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG66554 anti-NFYB antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then stained with ARG66554 anti-NFYB antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.