ARG66548
anti-NFYA antibody
anti-NFYA antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NFYA |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NFYA |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of Human NFYA. |
| 偶联标记 | Un-conjugated |
| 別名 | NF-YA; HAP2; Nuclear transcription factor Y subunit A; CBF-A; CAAT box DNA-binding protein subunit A; CBF-B; Nuclear transcription factor Y subunit alpha |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | 42 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 30% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P23511 Human Nuclear transcription factor Y subunit alpha Swiss-port # P23708 Mouse Nuclear transcription factor Y subunit alpha |
|---|---|
| 基因名称 | NFYA |
| 全名 | nuclear transcription factor Y, alpha |
| 背景介绍 | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008] |
| 生物功能 | Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 37 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG66548 anti-NFYA antibody ICC/IF image
Immunofluorescence: Formalin-fixed K562 cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66548 anti-NFYA antibody (red) in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. DAPI was used to stain the cell nuclei (blue).
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ARG66548 anti-NFYA antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then stained with ARG66548 anti-NFYA antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ARG66548 anti-NFYA antibody WB image
Western blot: A431, K562 and NIH/3T3 whole cell lysates stained with ARG66548 anti-NFYA antibody.
