ARG63152
anti-NFIL3 antibody
anti-NFIL3 antibody for Flow cytometry,Western blot and Human
Gene Regulation antibody; Immune System antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes NFIL3 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat |
| 应用 | FACS, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NFIL3 |
| 抗原物种 | Human |
| 抗原 | C-RLIATQPISASDSG |
| 偶联标记 | Un-conjugated |
| 別名 | Nuclear factor interleukin-3-regulated protein; NF-IL3A; E4BP4; IL3BP1; Transcriptional activator NF-IL3A; NFIL3A; Interleukin-3 promoter transcriptional activator; E4 promoter-binding protein 4; Interleukin-3-binding protein 1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q16649 Human Nuclear factor interleukin-3-regulated protein |
|---|---|
| 背景介绍 | Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).[supplied by OMIM, Feb 2009] |
| 研究领域 | Gene Regulation antibody; Immune System antibody |
| 预测分子量 | 51 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG63152 anti-NFIL3 antibody WB image
Western Blot: HepG2 lysate (RIPA buffer, 20 µg total protein per lane) stained with ARG63152 anti-NFIL3 antibody at 0.5 µg/ml dilution.
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ARG63152 anti-NFIL3 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed Human peripheral blood mononuclear cells permeabilized with 0.5% Triton. Cells were stained with ARG63152 anti-NFIL3 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
