ARG42939
anti-NFATc3 antibody
anti-NFATc3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NFATc3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NFATc3 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-290 of Human NFATc3 (NP_775188.1). |
| 偶联标记 | Un-conjugated |
| 別名 | NFAT4; NFATc3; NFATx; NFATX; NF-ATc3; Nuclear factor of activated T-cells, cytoplasmic 3; T-cell transcription factor NFAT4; NF-AT4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse thymus | ||||||||
| 实际分子量 | ~ 140 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q12968 Human Nuclear factor of activated T-cells, cytoplasmic 3 |
|---|---|
| 基因名称 | NFATC3 |
| 全名 | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 |
| 背景介绍 | The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2010] |
| 生物功能 | Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 (PubMed:18815128). Along with NFATC4, involved in embryonic heart development (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. [UniProt] |
| 预测分子量 | 116 kDa |
| 翻译后修饰 | Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG42939 anti-NFATc3 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG42939 anti-NFATc3 antibody.
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ARG42939 anti-NFATc3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon carcinoma tissue stained with ARG42939 anti-NFATc3 antibody at 1:200 dilution.
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ARG42939 anti-NFATc3 antibody WB image
Western blot: 25 µg of Mouse thymus lysate stained with ARG42939 anti-NFATc3 antibody at 1:500 dilution.
