ARG43375
anti-NFATc2 / NFAT1 phospho (Ser326) antibody
anti-NFATc2 / NFAT1 phospho (Ser326) antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NFATc2 / NFAT1 phospho (Ser326) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 特异性 | The antibody detects endogenous levels of NFAT1 only when phosphorylated at Ser326. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NFATc2 / NFAT1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated phosphospecific peptide around Ser326 of Human NFATc2 / NFAT1. |
| 偶联标记 | Un-conjugated |
| 別名 | NFATc2; NFATP; NFAT1; NFAT pre-existing subunit; NF-ATc2; T-cell transcription factor NFAT1; Nuclear factor of activated T-cells, cytoplasmic 2; NF-ATp |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Rat brain | ||||
| 实际分子量 | 100 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with phospho-specific peptide and the non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q13469 Human Nuclear factor of activated T-cells, cytoplasmic 2 Swiss-port # Q60591 Mouse Nuclear factor of activated T-cells, cytoplasmic 2 |
|---|---|
| 基因名称 | NFATC2 |
| 全名 | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 |
| 背景介绍 | This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012] |
| 生物功能 | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. [UniProt] |
| 预测分子量 | 100 kDa |
| 翻译后修饰 | In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity. Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG43375 anti-NFATc2 / NFAT1 phospho (Ser326) antibody WB image
Western blot: Rat brain lysate stained with ARG43375 anti-NFATc2 / NFAT1 phospho (Ser326) antibody.
