ARG51750
anti-NF2 / Merlin phospho (Ser518) antibody
anti-NF2 / Merlin phospho (Ser518) antibody for ICC/IF,Western blot and Human,Mouse,Rat
Cancer antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NF2 / Merlin phospho (Ser518) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NF2 / Merlin |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of serine 518 (R-L-S(p)-M-E) derived from Human Merlin. |
| 偶联标记 | Un-conjugated |
| 別名 | Merlin; Schwannomin; SCH; Schwannomerlin; ACN; Moesin-ezrin-radixin-like protein; BANF; Neurofibromin-2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NF2 |
| 全名 | neurofibromin 2 (merlin) |
| 背景介绍 | Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. |
| 生物功能 | Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. [UniProt] |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 70 kDa |
| 翻译后修饰 | Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail (PubMed:20178741). The dephosphorylation of Ser-518 favors the interaction with NOP53 (PubMed:21167305). Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation. |
检测图片 (3) Click the Picture to Zoom In
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ARG51750 anti-NF2 / Merlin phospho (Ser518) antibody WB image
Western blot: Extracts from HUVEC cells untreated(lane 1) or treated with IFN-a(lane 2) stained with ARG51750 anti-NF2 / Merlin phospho (Ser518) antibody.
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ARG51750 anti-NF2 / Merlin phospho (Ser518) antibody WB image
Western blot: Extracts from C6 cells, treated with calf intestinal phosphatase (CIP), stained with ARG51750 anti-NF2 / Merlin phospho (Ser518) antibody.
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ARG51750 anti-NF2 / Merlin phospho (Ser518) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51750 anti-NF2 / Merlin phospho (Ser518) antibody.
