ARG40446
anti-NEK3 antibody
anti-NEK3 antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NEK3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NEK3 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 267-506 of Human NEK3 (NP_689933.1). |
| 偶联标记 | Un-conjugated |
| 別名 | HSPK36; Never in mitosis A-related kinase 3; HSPK 36; Serine/threonine-protein kinase Nek3; EC 2.7.11.1; NimA-related protein kinase 3 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | HeLa | ||||
| 实际分子量 | 55 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P51956 Human Serine/threonine-protein kinase Nek3 Swiss-port # Q9R0A5 Mouse Serine/threonine-protein kinase Nek3 |
|---|---|
| 基因名称 | NEK3 |
| 全名 | NIMA-related kinase 3 |
| 背景介绍 | This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009] |
| 生物功能 | Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2. [UniProt] |
| 细胞定位 | Cytoplasm. Cell projection, axon. [UniProt] |
| 预测分子量 | 58 kDa |
| 翻译后修饰 | Phosphorylation at Thr-479 regulates its catalytic activity. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG40446 anti-NEK3 antibody WB image
Western blot: 25 µg of HeLa cell lysate stained with ARG40446 anti-NEK3 antibody at 1:1000 dilution.
