ARG55793
anti-NEDD4 antibody
anti-NEDD4 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NEDD4 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NEDD4 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 1288-1319 (C-terminus) of Human NEDD4. |
| 偶联标记 | Un-conjugated |
| 別名 | NEDD-4; EC 6.3.2.-; RPF1; E3 ubiquitin-protein ligase NEDD4; NEDD4-1; Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | NIH/3T3 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NEDD4 |
| 全名 | neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase |
| 生物功能 | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. The direct link between NEDD4 and PTEN regulation through polyubiquitination is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane; Peripheral membrane protein Note=Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1. |
| 预测分子量 | 149 kDa |
| 翻译后修饰 | Auto-ubiquitinated. |
检测图片 (3) Click the Picture to Zoom In
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ARG55793 anti-NEDD4 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded brain tissue stained with ARG55793 anti-NEDD4 antibody.
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ARG55793 anti-NEDD4 antibody WB image
Western blot: 35 µg of NIH/3T3 cell lysate stained with ARG55793 anti-NEDD4 antibody.
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ARG55793 anti-NEDD4 antibody FACS image
Flow Cytometry: NCI-H460 cells stained with ARG55793 anti-NEDD4 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC-labelled secondary antibody.
