ARG65574
anti-NDUFS2 antibody
anti-NDUFS2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NDUFS2 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NDUFS2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of human NDUFS2 |
| 偶联标记 | Un-conjugated |
| 別名 | Complex I-49kD; CI-49kD; CI-49; NADH-ubiquinone oxidoreductase 49 kDa subunit; EC 1.6.99.3; NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; EC 1.6.5.3 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse heart and brain tissue, Jurkat cell |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by antigen-affinity chromatography. |
| 缓冲液 | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 40% Glycerol |
| 浓度 | 3.1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O75306 Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Swiss-port # Q91WD5 Mouse NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial |
|---|---|
| 基因名称 | NDUFS2 |
| 全名 | NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) |
| 背景介绍 | The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| 生物功能 | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). [UniProt] |
| 产品亮点 | Related products: NDUFS2 antibodies; Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 53 kDa |
| 翻译后修饰 | Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex (PubMed:24089531, PubMed:24838397). |
检测图片 (3) Click the Picture to Zoom In
-
ARG65574 anti-NDUFS2 antibody WB image
Western blot: 40 μg of 1) Mouse heart, 2) Mouse brain and 3) Jurkat lysates stained with ARG65574 anti-NDUFS2 antibody at 1:400 dilution. Exposure time: 20 seconds.
-
ARG65574 anti-NDUFS2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue stained with ARG65574 anti-NDUFS2 antibody (left) at 1:50 dilution, or the same antibody preincubated with synthetic peptide (right). (Original magnification: ×200).
-
ARG65574 anti-NDUFS2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue stained with ARG65574 anti-NDUFS2 antibody (left) at 1:50 dilution, or the same antibody preincubated with synthetic peptide (right). (Original magnification: ×200).
