ARG45919

anti-NDUFS1 antibody

anti-NDUFS1 antibody for Flow cytometry,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes NDUFS1
反应物种 Hu, Ms, Rat
应用 FACS, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 NDUFS1
抗原物种 Human
抗原 Recombinant protein containing to human NDUFS1.
偶联标记 Un-conjugated
別名 NDUFS1; NADH:Ubiquinone Oxidoreductase Core Subunit S1; CI-75k; EC 1.6.5.3; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; CI-75Kd; EC 1.6.99.3; PRO1304; Complex I-75kD; CI-75kD

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 79 kDa

属性

形式 Liquid
纯化 Affinity chromatography purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 227197 Mouse NDUFS1

GeneID: 301458 Rat NDUFS1

GeneID: 4719 Human NDUFS1

基因名称 NDUFS1
全名 NADH:Ubiquinone Oxidoreductase Core Subunit S1
背景介绍 The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
生物功能 Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. [UniProt]
细胞定位 Membrane; Mitochondrion; Mitochondrion inner membrane. [UniProt]
预测分子量 79 kDa
翻译后修饰 Acetylation. [UniProt]

检测图片 (4) Click the Picture to Zoom In

  • ARG45919 anti-NDUFS1 antibody WB image

    Western blot: 293T stained with ARG45919 anti-NDUFS1 antibody at 0.5 μg/ml dilution.

  • ARG45919 anti-NDUFS1 antibody FACS image

    Flow Cytometry: HepG2 stained with ARG45919 anti-NDUFS1 antibody at 1 µg/10^6 cells dilution.

  • ARG45919 anti-NDUFS1 antibody WB image

    Western blot: Rat kidney stained with ARG45919 anti-NDUFS1 antibody at 0.5 μg/ml dilution.

  • ARG45919 anti-NDUFS1 antibody WB image

    Western blot: Mouse kidney stained with ARG45919 anti-NDUFS1 antibody at 0.5 μg/ml dilution.