ARG43935

anti-NDUFA5 antibody

anti-NDUFA5 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes NDUFA5
反应物种 Hu, Ms, Rat
应用 ELISA, FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 NDUFA5
抗原物种 Human
抗原 Human NDUFA5 recombinant protein
表达系统 E.coli
偶联标记 Un-conjugated
Protein Full name NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
別名 NDUFA5; NADH:Ubiquinone Oxidoreductase Subunit A5; NADH-Ubiquinone Oxidoreductase 13 KDa-B Subunit; CI-13KD-B; CI-13kB; UQOR13; NUFM; B13; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 5, 13kDa; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Subunit 5; Complex I 13kDa Subunit B; Complex I Subunit B13; Ubiquinone Reductase; Type I Dehydrogenase; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 5 (13kD, B13); NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 5; Complex I-13kD-B; CI-13kD-B

应用说明

应用建议
应用 推荐稀释比
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified with Immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 25488 Rat NDUFA5

GeneID: 4698 Human NDUFA5

GeneID: 68202 Mouse NDUFA5

基因名称 NDUFA5
全名 NADH:Ubiquinone Oxidoreductase Subunit A5
背景介绍 This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16.
生物功能 Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
细胞定位 Membrane, Mitochondrion, Mitochondrion inner membrane
预测分子量 13 kDa
翻译后修饰 Acetylation

检测图片 (5) Click the Picture to Zoom In

  • ARG43935 anti-NDUFA5 antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG43935 anti-NDUFA5 antibody at 5 μg/ml dilution.

  • ARG43935 anti-NDUFA5 antibody WB image

    Western blot: Rat heart stained with ARG43935 anti-NDUFA5 antibody at 0.5 μg/mL dilution.

  • ARG43935 anti-NDUFA5 antibody FACS image

    Flow Cytometry: U937 cells stained with ARG43935 anti-NDUFA5 antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43935 anti-NDUFA5 antibody WB image

    Western blot: Hela and K562 stained with ARG43935 anti-NDUFA5 antibody at 0.5 μg/mL dilution.

  • ARG43935 anti-NDUFA5 antibody WB image

    Western blot: Mouse heart stained with ARG43935 anti-NDUFA5 antibody at 0.5 μg/mL dilution.