ARG57159
anti-NDRG1 antibody [11G4]
anti-NDRG1 antibody [11G4] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [11G4] recognizes NDRG1 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 11G4 |
| 同位型 | IgG1, kappa |
| 靶点名称 | NDRG1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-394 of Human NDRG1 |
| 偶联标记 | Un-conjugated |
| 別名 | DRG1; Protein NDRG1; Differentiation-related gene 1 protein; PROXY1; TARG1; RIT42; Reducing agents and tunicamycin-responsive protein; Rit42; CMT4D; RTP; CAP43; N-myc downstream-regulated gene 1 protein; TDD5; HMSNL; NMSL; Nickel-specific induction protein Cap43; NDR1; DRG-1; GC4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NDRG1 |
| 全名 | N-myc downstream regulated 1 |
| 背景介绍 | This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
| 生物功能 | Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy. [UniProt] |
| 预测分子量 | 43 kDa |
| 翻译后修饰 | Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SGK1 is cell cycle dependent. |
检测图片 (1) Click the Picture to Zoom In
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ARG57159 anti-NDRG1 antibody [11G4] WB image
Western blot: 40 µg of 1) LnCaP, 2) HeLa, 3) PC3, 4) Hep3B, 5) HT1080, 6) Jurkat, and 7) SW480 cell lysates stained with ARG57159 anti-NDRG1 antibody [11G4] at 1:1000.
