ARG45411

anti-NDFIP2 antibody [10D6D7]

anti-NDFIP2 antibody [10D6D7] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

概述

产品描述 Mouse Monoclonal antibody [10D6D7] recognizes NDFIP2
反应物种 Hu
应用 FACS, ICC/IF, IHC-P, WB
宿主 Mouse
克隆 Monoclonal
克隆号 10D6D7
同位型 IgG2b
靶点名称 NDFIP2
抗原物种 Human
抗原 Recombinant protein containing to human NDFIP2.
偶联标记 Un-conjugated
別名 NDFIP2; Nedd4 Family Interacting Protein 2; KIAA1165; N4wbp5a; Putative MAPK-Activating Protein PM04/PM05/PM06/PM07; Putative NF-Kappa-B-Activating Protein 413; NEDD4 Family-Interacting Protein 2; NEDD4 WW Domain-Binding Protein 5A; MAPK-Activating Protein PM04 PM05 PM06 PM07; NF-Kappa-B-Activating Protein 413; N4WBP5A

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 39 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 54602 Human NDFIP2

Swiss-port # Q9NV92 Human NEDD4 family-interacting protein 2

基因名称 NDFIP2
全名 Nedd4 Family Interacting Protein 2
背景介绍 Enables WW domain binding activity. Involved in negative regulation of gene expression; negative regulation of transport; and positive regulation of protein ubiquitination. Located in several cellular components, including Golgi apparatus; mitochondrion; and perinuclear region of cytoplasm. [provided by Alliance of Genome Resources, Nov 2024]
生物功能 Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus and multivesicular body where it mediates KCNH2 degradation. [UniProt]
细胞定位 Endosome; Golgi apparatus; Membrane. [UniProt]
预测分子量 36 kDa
翻译后修饰 Phosphoprotein; Ubl conjugation. [UniProt]

检测图片 (4) Click the Picture to Zoom In

  • ARG45411 anti-NDFIP2 antibody [10D6D7] IHC-P image

    Immunohistochemistry: Human placenta stained with ARG45411 anti-NDFIP2 antibody [10D6D7] at 2 μg/ml dilution.

  • ARG45411 anti-NDFIP2 antibody [10D6D7] ICC/IF image

    Immunofluorescence: A431 stained with ARG45411 anti-NDFIP2 antibody [10D6D7] at 5 μg/ml dilution.

  • ARG45411 anti-NDFIP2 antibody [10D6D7] WB image

    Western blot: A549 stained with ARG45411 anti-NDFIP2 antibody [10D6D7] at 0.5 μg/ml dilution.

  • ARG45411 anti-NDFIP2 antibody [10D6D7] FACS image

    Flow Cytometry: JK stained with ARG45411 anti-NDFIP2 antibody [10D6D7] at 1 µg/10^6 cells dilution.