ARG57081
anti-NCS1 antibody [11B8]
anti-NCS1 antibody [11B8] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [11B8] recognizes NCS1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 11B8 |
| 同位型 | IgG1, kappa |
| 靶点名称 | NCS1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-190 of Human NCS1. |
| 偶联标记 | Un-conjugated |
| 別名 | Frequenin homolog; NCS-1; Frequenin-like protein; Neuronal calcium sensor 1; Frequenin-like ubiquitous protein; FLUP; FREQ |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NCS1 |
| 全名 | neuronal calcium sensor 1 |
| 背景介绍 | This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel (By similarity). [UniProt] |
| 预测分子量 | 22 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG57081 anti-NCS1 antibody [11B8] FACS image
Flow Cytometry: A549 cell line stained with ARG57081 anti-NCS1 antibody [11B8] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57081 anti-NCS1 antibody [11B8] WB image
Western blot: 40 µg of 1) 293T cell lysate, 2) K562 cell lysate, 3) LaCap cell lysate, 4) HeLa cell lysate stained with ARG57081 anti-NCS1 antibody [11B8] at 1:1000.
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ARG57081 anti-NCS1 antibody [11B8] FACS image
Flow Cytometry: HeLa cell line stained with ARG57081 anti-NCS1 antibody [11B8] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
