ARG59810
anti-NCK2 antibody
anti-NCK2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes NCK2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NCK2 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-380 of Human NCK2 (NP_003572.2). |
| 偶联标记 | Un-conjugated |
| 別名 | Nck-2; NCKbeta; NCK adaptor protein 2; SH2/SH3 adaptor protein NCK-beta; GRB4; Growth factor receptor-bound protein 4; Cytoplasmic protein NCK2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Rat brain, Mouse lung and 293T | ||||||
| 实际分子量 | 43 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NCK2 |
| 全名 | NCK adaptor protein 2 |
| 背景介绍 | This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| 生物功能 | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. [UniProt] |
| 细胞定位 | Cytoplasm. Endoplasmic reticulum. [UniProt] |
| 预测分子量 | 43 kDa |
| 翻译后修饰 | Phosphorylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG59810 anti-NCK2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate stained with ARG59810 anti-NCK2 antibody at 1:100 dilution.
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ARG59810 anti-NCK2 antibody WB image
Western blot: 25 µg of Rat brain, Mouse lung and 293T cell lysates stained with ARG59810 anti-NCK2 antibody at 1:1000 dilution.
