ARG57025
anti-NBL1 antibody [38G8]
anti-NBL1 antibody [38G8] for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [38G8] recognizes NBL1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 38G8 |
| 同位型 | IgG1, kappa |
| 靶点名称 | NBL1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 18-181 of Human NBL1. |
| 偶联标记 | Un-conjugated |
| 別名 | NB; DAN; NO3; DAND1; D1S1733E |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P41271 Human Neuroblastoma suppressor of tumorigenicity 1 |
|---|---|
| 基因名称 | NBL1 |
| 全名 | neuroblastoma 1, DAN family BMP antagonist |
| 背景介绍 | This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013] |
| 生物功能 | Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. [UniProt] |
| 预测分子量 | 19 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG57025 anti-NBL1 antibody [38G8] ICC/IF image
Immunoflorescense: Jurkat cell line stained with ARG57025 anti-NBL1 antibody [38G8] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57025 anti-NBL1 antibody [38G8] FACS image
Flow Cytometry: Jurkat cell line stained with ARG57025 anti-NBL1 antibody [38G8] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57025 anti-NBL1 antibody [38G8] WB image
Western blot: 30 µg of Jurkat cell lysate stained with ARG57025 anti-NBL1 antibody [38G8] at 1:1000.
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ARG57025 anti-NBL1 antibody [38G8] ICC/IF image
Immunoflorescense: A549 cell line stained with ARG57025 anti-NBL1 antibody [38G8] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57025 anti-NBL1 antibody [38G8] FACS image
Flow Cytometry: A549 cell line stained with ARG57025 anti-NBL1 antibody [38G8] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
