ARG64194
anti-NANOG antibody
anti-NANOG antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Developmental Biology antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes NANOG |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | NANOG |
| 抗原物种 | Human |
| 抗原 | C-DLFLNYSMNMQPED |
| 偶联标记 | Un-conjugated |
| 別名 | Homeobox transcription factor Nanog; Homeobox protein NANOG; hNanog |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NANOG |
| 全名 | Nanog homeobox |
| 背景介绍 | The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| 生物功能 | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. [UniProt] |
| 产品亮点 | Related products: NANOG antibodies; Anti-Goat IgG secondary antibodies; Related news: Stem cell and the regenerative medicine: Ready for the patients |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Gene Regulation antibody |
| 预测分子量 | 35 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG64194 anti-NANOG antibody WB image
Western Blot: HeLa (nuclear) cell lysate (35 µg protein in RIPA buffer) stained with ARG64194 anti-NANOG antibody at 0.05 µg/ml dilution.
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ARG64194 anti-NANOG antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Testis. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64194 anti-NANOG antibody at 2 µg/ml dilution followed by AP-staining.
